3EX7
The crystal structure of EJC in its transition state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91745 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 182.040, 100.750, 145.880 |
Unit cell angles | 90.00, 112.08, 90.00 |
Refinement procedure
Resolution | 39.065 - 2.301 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hyi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.215 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BEAST |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 107635 | |
<I/σ(I)> | 11.35 | 2.58 |
Completeness [%] | 99.1 | 96 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 7% PEG 3350, 50mM Tris HCl pH 8.8, 200mM NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | NaAcetate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 3350 | ||
6 | 1 | 2 | Tris | ||
7 | 1 | 2 | NaAcetate | ||
8 | 1 | 2 | H2O |