3EW8

Crystal Structure Analysis of human HDAC8 D101L variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone deacetylase 8	polymer	388	43173.0	1	UniProt (Q9BY41) Pfam (PF00850)	Homo sapiens (Human)	HD8
2	B, D (A)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	E (A)	4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl]benzamide	non-polymer		307.4	1	Chemie (B3N)
5	F (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
6	G, H, I (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	J (A)	water	water		18.0	253	Chemie (HOH)

Sequence modifications

A: 1 - 377 (UniProt: Q9BY41)

PDB	External Database	Details
Leu 101	Asp 101	engineered mutation
Ile 378	-	expression tag
Glu 379	-	expression tag
Gly 380	-	expression tag
Arg 381	-	expression tag
Ser 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43173.0
Non-Polymers*	Number of molecules	8
	Total formula weight	805.4
All*	Total formula weight	43978.4

*Water molecules are not included.