3ETB

Crystal structure of the engineered neutralizing antibody M18 complexed with anthrax protective antigen domain 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (F, G, H, I)	Antibody M18 light chain and antibody M18 heavy chain linked with a synthetic (GGGGS)4 linker	polymer	252	26866.6	4		Mus musculus (mouse)
2	E, F, G, H (J, K, L, M)	Anthrax Protective Antigen	polymer	144	16499.6	4	UniProt (P13423) Pfam (PF20835)	Bacillus anthracis	PA, PA-83, PA83, Anthrax toxins translocating protein [Contains: Protective antigen PA-20 and Protective antigen PA-63]

Sequence modifications

F, G, H, I: 1 - 108 (PDB: 3ETB)

PDB	External Database	Details
Met -4	-	expression tag
Ala -3	-	expression tag
Asp -2	-	expression tag
Tyr -1	-	expression tag
Lys 0	-	expression tag
Val 21	Ile 21	engineered mutation
Phe 46	Leu 46	engineered mutation
Pro 56	Ser 56	engineered mutation
Asn 76	Ser 76	engineered mutation
Leu 78	Gln 78	engineered mutation
Pro 94	Leu 94	engineered mutation

F, G, H, I: 1001 - 1113 (PDB: 3ETB)

PDB	External Database	Details
Gly 109	-	linker
Gly 110	-	linker
Gly 111	-	linker
Gly 112	-	linker
Ser 113	-	linker
Gly 114	-	linker
Gly 115	-	linker
Gly 116	-	linker
Gly 117	-	linker
Ser 118	-	linker
Gly 119	-	linker
Gly 120	-	linker
Gly 121	-	linker
Gly 122	-	linker
Ser 123	-	linker
Gly 124	-	linker
Gly 125	-	linker
Gly 126	-	linker
Gly 127	-	linker
Ser 128	-	linker
Asn 1030	Ser 30	engineered mutation
Ser 1057	Thr 58	engineered mutation
Glu 1064	Lys 65	engineered mutation
Ile 1068	Thr 69	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	173464.7
All*	Total formula weight	173464.7

*Water molecules are not included.