3ERR

Microtubule binding domain from mouse cytoplasmic dynein as a fusion with seryl-tRNA synthetase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	fusion protein of microtubule binding domain from mouse cytoplasmic dynein and seryl-tRNA synthetase from Thermus thermophilus	polymer	536	60957.7	2	UniProt (Q9JHU4) UniProt (P34945) Pfam (PF12777) Pfam (PF00587) UniProt (by SIFTS) (Q5SJX7)	Mus musculus	Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, Seryl-tRNA(Ser/Sec) synthetase, Serine-tRNA ligase, SerRS
2	C, D (A, B)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	E, F (A, B)	water	water		18.0	123	Chemie (HOH)

Sequence modifications

A, B: 3260 - 3427 (UniProt: Q9JHU4)

PDB	External Database	Details
Met 1	-	expression tag
Val 2	-	expression tag
Asp 3	-	expression tag
Leu 4	-	expression tag
Lys 5	-	expression tag
Arg 6	-	expression tag
Leu 7	-	expression tag
Arg 8	-	expression tag
Gln 9	-	expression tag
Glu 10	-	expression tag
Pro 11	-	expression tag
Glu 12	-	expression tag
Val 13	-	expression tag
Phe 14	-	expression tag
His 15	-	expression tag
Arg 16	-	expression tag
Ala 17	-	expression tag
Ile 18	-	expression tag
Arg 19	-	expression tag
Glu 20	-	expression tag
Lys 21	-	expression tag
Gly 22	-	expression tag
Val 23	-	expression tag
Ala 24	-	expression tag
Leu 25	-	expression tag
Asp 26	-	expression tag
Leu 27	-	expression tag
Glu 28	-	expression tag
Ala 29	-	expression tag
Leu 30	-	expression tag
Leu 31	-	expression tag
Ala 32	-	expression tag
Val 33	-	expression tag
Asp 34	-	expression tag
Ala 3323	Cys 3323	engineered mutation
Ala 3387	Cys 3387	engineered mutation
Gly 420	-	expression tag
Ala 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag

A, B: 94 - 419 (UniProt: P34945)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121915.5
Non-Polymers*	Number of molecules	2
	Total formula weight	694.4
All*	Total formula weight	122609.9

*Water molecules are not included.