3EO3

Crystal structure of the N-acetylmannosamine kinase domain of human GNE protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	polymer	333	35684.9	3	UniProt (Q9Y223) Pfam (PF00480)	Homo sapiens (Human)	UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, UDP-GlcNAc-2-epimerase, N-acetylmannosamine kinase, ManAc kinase
2	D, G, I (A, B, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
3	E, F, H, J (A, B, C)	UNKNOWN ATOM OR ION	non-polymer			4	Chemie (UNX)

Sequence modifications

A, B, C: 406 - 720 (UniProt: Q9Y223)

PDB	External Database	Details
Met 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
Ser 395	-	expression tag
Ser 396	-	expression tag
Gly 397	-	expression tag
Arg 398	-	expression tag
Glu 399	-	expression tag
Asn 400	-	expression tag
Leu 401	-	expression tag
Tyr 402	-	expression tag
Phe 403	-	expression tag
Gln 404	-	expression tag
Gly 405	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	107054.8
Non-Polymers*	Number of molecules	7
	Total formula weight	196.2
All*	Total formula weight	107251.0

*Water molecules are not included.