3EF7

ZP-N domain of mammalian sperm receptor ZP3 (crystal form III)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Maltose-binding periplasmic protein, LINKER, Zona pellucida protein 3	polymer	481	52948.9	2	UniProt (P0AEX9) UniProt (P10761) Pfam (PF01547) Pfam (PF00100) In PDB	Escherichia coli (strain K12)	Maltose-binding protein/ZP3 ZP-N domain chimera, MBP, Zona pellucida glycoprotein ZP3, Sperm receptor, Zona pellucida protein C
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A, B	ZINC ION	non-polymer		65.4	13	Chemie (ZN)
4	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 2 - 368 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 3	Ile 28	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	Lys 388	engineered mutation
Ala 364	Asp 389	engineered mutation
Asn 368	Arg 393	engineered mutation

A, B: 372 - 473 (UniProt: P10761)

PDB	External Database	Details
Leu 474	-	expression tag
Glu 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag

A, B: 369 - 371 (PDB: 3EF7)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105897.9
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	17
	Total formula weight	1010.6
All*	Total formula weight	107593.1

*Water molecules are not included.