3EF7
ZP-N domain of mammalian sperm receptor ZP3 (crystal form III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.28235 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.200, 98.580, 143.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.160 - 3.100 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT AND ZN-SINGLE-WAVELENGTH ANOMALOUS DIFFRACTION |
Starting model (for MR) | 3d4g |
RMSD bond length | 0.011 |
RMSD bond angle | 1.039 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 3.170 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 42898 | |
<I/σ(I)> | 17.7 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | Sample: 15mg/ml protein in 0.05M sodium chloride, 0.01M Tris-HCL, PH7.2, 0.001M Maltose. Reservoir: 12% PEG6000, 0.15M calcium chloride, 0.0025M zinc chloride, 0.1M Tris-HCL, PH8.2. Sample to reservoir ratio in drop: 1:1, PH8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K, pH7.8 |