3EE7

Crystal Structure of SARS-CoV nsp9 G104E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Replicase polyprotein 1a	polymer	121	13558.3	4	UniProt (P0C6U8) Pfam (PF08710)	SARS coronavirus (SARS-CoV)	pp1a, ORF1a polyprotein, Non-structural protein 1, nsp1, Leader protein, Non-structural protein 2, nsp2, p65 homolog, Non-structural protein 3, nsp3, Papain-like proteinase, PL-PRO, PL2-PRO, Non-structural protein 4, nsp4, 3C-like proteinase, 3CL-PRO, 3CLp, nsp5, Non-structural protein 6, nsp6, Non-structural protein 7, nsp7, Non-structural protein 8, nsp8, Non-structural protein 9, nsp9, Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Non-structural protein 11, nsp11
2	E, F, G, M, P... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
3	AA, BA, H, I, J... (D, A, B, C)	GLYCEROL	non-polymer		92.1	16	Chemie (GOL)
4	CA, DA, EA, FA (A, B, C, D)	water	water		18.0	116	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 113 (UniProt: P0C6U8)

PDB	External Database	Details
Glu 104	Gly 4221	engineered mutation
Leu 114	-	expression tag
Glu 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	54233.4
Non-Polymers*	Number of molecules	24
	Total formula weight	2233.3
All*	Total formula weight	56466.7

*Water molecules are not included.