3EE5
Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C | Beta-1,4-galactosyltransferase 1 | polymer | 287 | 32773.2 | 3 | UniProt (P15291) Pfam (PF13733) Pfam (PF02709) In PDB | Homo sapiens (Human) | Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 |
| 2 | D, E, F | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose | branched | 383.3 | 3 | In PDB GlyTouCan (G33476GF) | |||
| 3 | C, A, B | 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE | non-polymer | 503.3 | 3 | Chemie (UDH) | |||
| 4 | C, A, B | naphthalen-2-ylmethanol | non-polymer | 158.2 | 3 | Chemie (2NA) | |||
| 5 | B, A, C | SULFATE ION | non-polymer | 96.1 | 14 | Chemie (SO4) | |||
| 6 | B, C, A | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
| 7 | C, A, B | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 8 | C | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 1 | Chemie (DIO) | |||
| 9 | C | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 10 | water | water | 18.0 | 475 | Chemie (HOH) |
Sequence modifications
A, B, C: 126 - 398 (UniProt: P15291)
| PDB | External Database | Details |
|---|---|---|
| Ala 112 | - | expression tag |
| Ser 113 | - | expression tag |
| Met 114 | - | expression tag |
| Thr 115 | - | expression tag |
| Gly 116 | - | expression tag |
| Gly 117 | - | expression tag |
| Gln 118 | - | expression tag |
| Gln 119 | - | expression tag |
| Met 120 | - | expression tag |
| Gly 121 | - | expression tag |
| Arg 122 | - | expression tag |
| Gly 123 | - | expression tag |
| Ser 124 | - | expression tag |
| Ala 125 | - | expression tag |
| Thr 337 | Arg 337 | engineered mutation |
| Thr 338 | Cys 338 | engineered mutation |
| His 340 | Met 340 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98319.7 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1150.0 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 4422.3 | |
| All* | Total formula weight | 103892.0 |
