3E9J

Structure of the charge-transfer intermediate of the transmembrane redox catalyst DsbB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, E	Thiol/disulfide oxidoreductase DsbA	polymer	189	21123.0	2	UniProt (P0AEG4) Pfam (PF01323) In PDB	Escherichia coli K12	Thiol:disulfide interchange protein dsbA
2	C, F	Thiol/disulfide oxidoreductase DsbB	polymer	182	20951.9	2	UniProt (P0A6M2) Pfam (PF02600) In PDB	Escherichia coli K12	Disulfide bond formation protein B
3	C, F	UBIQUINONE-1	non-polymer		250.3	2	Chemie (UQ1)

Sequence modifications

B, E: 1 - 189 (UniProt: P0AEG4)

PDB	External Database	Details
Ala 33	Cys 52	engineered mutation

C, F: 1 - 176 (UniProt: P0A6M2)

PDB	External Database	Details
Ala 8	Cys 8	engineered mutation
Val 49	Cys 49	engineered mutation
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	84149.8
Non-Polymers*	Number of molecules	2
	Total formula weight	500.6
All*	Total formula weight	84650.4

*Water molecules are not included.