3E7J
HeparinaseII H202A/Y257A double mutant complexed with a heparan sulfate tetrasaccharide substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Heparinase II protein | polymer | 749 | 84816.5 | 2 | UniProt (Q46080) Pfam (PF16332) Pfam (PF07940) Pfam (PF18675) UniProt (by SIFTS) (C6XZB6) | Pedobacter heparinus | |
| 2 | C, D (C, D) | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranuronic acid-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 758.6 | 2 | In PDB GlyTouCan (G74165GY) | |||
| 3 | E, J (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | F, G, H, I, K (A, B) | ACETATE ION | non-polymer | 59.0 | 5 | Chemie (ACT) | |||
| 5 | L, M (A, B) | water | water | 18.0 | 782 | Chemie (HOH) |
Sequence modifications
A, B: 24 - 772 (UniProt: Q46080)
| PDB | External Database | Details |
|---|---|---|
| Ala 202 | His 202 | engineered mutation |
| Ala 257 | Tyr 257 | engineered mutation |
| Ala 758 | Pro 758 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 169633.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1517.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 426.0 | |
| All* | Total formula weight | 171576.4 |
