3E4Z

Crystal structure of human insulin degrading enzyme in complex with insulin-like growth factor II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Insulin-degrading enzyme	polymer	990	114558.7	2	UniProt (Q5T5N2) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) UniProt (by SIFTS) (P14735)	Homo sapiens (human)	Insulin protease,Insulinase,Insulysin
2	C, D (C, D)	Insulin-like growth factor II	polymer	67	7484.5	2	UniProt (P01344) Pfam (PF00049)	Homo sapiens (human)	IGF-II,Somatomedin-A
3	E, F (B, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H, I (A, B, C)	water	water		18.0	724	Chemie (HOH)

Sequence modifications

A, B: 42 - 1019 (UniProt: Q5T5N2)

PDB	External Database	Details
Met 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	expression tag
Ala 38	-	expression tag
Gly 39	-	expression tag
Ile 40	-	expression tag
Pro 41	-	expression tag
Leu 110	Cys 110	engineered mutation
Gln 111	Glu 111	engineered mutation
Ser 171	Cys 171	engineered mutation
Ala 178	Cys 178	engineered mutation
Val 257	Cys 257	engineered mutation
Leu 414	Cys 414	engineered mutation
Asn 573	Cys 573	engineered mutation
Ser 590	Cys 590	engineered mutation
Ser 789	Cys 789	engineered mutation
Ala 812	Cys 812	engineered mutation
Ala 819	Cys 819	engineered mutation
Ser 904	Cys 904	engineered mutation
Tyr 908	Trp 908	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	244086.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	244217.1

*Water molecules are not included.