3E4Z

Crystal structure of human insulin degrading enzyme in complex with insulin-like growth factor II

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2007-01-01
Detector	MAR scanner 345 mm plate
Spacegroup name	P 65
格子定数 [Å]	263.033, 263.033, 90.816
格子定数 [度]	90.00, 90.00, 120.00

精密化法

分解能	50.000 - 2.280
R因子	0.19746
Rwork	0.196
R-free	0.22983
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.336
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.360
分解能 [Å] (高)	2.280	2.280
Rmerge_l_obs	0.134	0.507
独立反射数	162414
<I/σ(I)>	20.1	2.1
完全性 [%]	99.9	99.9
冗長性	5.4	3.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	298	10-13% PEG MME 5000, 100 mM HEPES pH 7.0, 4-14% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K