3E2N

Engineering ascorbate peroxidase activity into cytochrome c peroxidase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytochrome c peroxidase	polymer	287	32602.5	1	UniProt (P00431) UniProt (P48534) Pfam (PF00141) In PDB	Saccharomyces cerevisiae	CCP
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3		water	water		18.0	589	Chemie (HOH)

Sequence modifications

A: 1 - 29 (UniProt: P00431)
A: 30 - 35 (UniProt: P48534)
A: 36 - 287 (UniProt: P00431)

PDB	External Database	Details
Arg 177	Asn 251	engineered mutation
Phe 184	Trp 258	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32602.5
Non-Polymers*	Number of molecules	1
	Total formula weight	616.5
All*	Total formula weight	33219.0

*Water molecules are not included.