3E2E
Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase | polymer | 889 | 99829.2 | 1 | UniProt (P00573) Pfam (PF14700) Pfam (PF00940) | Bacteriophage T7 | |
| 2 | B (R) | RNA (5'-R(*GP*GP*GP*AP*GP*UP*G)-3') | polymer | 7 | 2316.4 | 1 | synthetic construct | ||
| 3 | C (T) | DNA (31-MER) | polymer | 33 | 10121.5 | 1 | synthetic construct | ||
| 4 | D (N) | DNA (28-MER) | polymer | 33 | 10080.5 | 1 | synthetic construct | ||
| 5 | E, F, G, H (A, R, T, N) | water | water | 18.0 | 73 | Chemie (HOH) |
Sequence modifications
A: 1 - 883 (UniProt: P00573)
| PDB | External Database | Details |
|---|---|---|
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Leu 266 | Pro 266 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 122347.6 | |
| All* | Total formula weight | 122347.6 |
