3E2E
Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.008, 81.008, 358.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.800 - 3.000 |
| R-factor | 0.24296 |
| Rwork | 0.240 |
| R-free | 0.29162 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.181 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.081 | 0.052 | 0.620 |
| Number of reflections | 27490 | ||
| Completeness [%] | 93.6 | 98.7 | 49.4 |
| Redundancy | 6.4 | 6.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 285 | PEG 6000, MgCl2, Tris ph8.0 at 285K, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 2 | PEG 6000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 1 | Tris | ||
| 6 | 1 | 2 | Tris |
