3E1F

E.Coli (lacZ) beta-galactosidase (H418E) in complex with galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1, 2, 3, 4	Beta-galactosidase	polymer	1023	116497.2	4	UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB	Escherichia coli K12	Lactase
2	4, 1, 2, 3	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
3	1, 2, 3, 4	SODIUM ION	non-polymer		23.0	13	Chemie (NA)
4	1, 2, 3, 4	DIMETHYL SULFOXIDE	non-polymer		78.1	73	Chemie (DMS)
5	1, 2, 3, 4	beta-D-galactopyranose	non-polymer		180.2	8	Chemie (GAL)
6		water	water		18.0	328	Chemie (HOH)

Sequence modifications

1, 2, 3, 4: 9 - 1023 (UniProt: P00722)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Leu 5	-	expression tag
Glu 6	-	expression tag
Asp 7	-	expression tag
Pro 8	-	expression tag
Glu 418	His 419	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	465988.9
Non-Polymers*	Number of molecules	104
	Total formula weight	7686.9
All*	Total formula weight	473675.8

*Water molecules are not included.