3E1F
E.Coli (lacZ) beta-galactosidase (H418E) in complex with galactose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (1, 2, 3, 4) | Beta-galactosidase | polymer | 1023 | 116497.2 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli K12 | Lactase |
| 2 | CD, E, F, G, KA... (4, 1, 2, 3) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) | |||
| 3 | H, I, J, NA, OA... (1, 2, 3, 4) | SODIUM ION | non-polymer | 23.0 | 13 | Chemie (NA) | |||
| 4 | AA, AB, AC, AD, BA... (1, 2, 3, 4) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 73 | Chemie (DMS) | |||
| 5 | FA, GA, HB, IB, OC... (1, 2, 3, 4) | beta-D-galactopyranose | non-polymer | 180.2 | 8 | Chemie (GAL) | |||
| 6 | ED, FD, GD, HD (1, 2, 3, 4) | water | water | 18.0 | 328 | Chemie (HOH) |
Sequence modifications
1, 2, 3, 4: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Glu 418 | His 419 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465988.9 | |
| Non-Polymers* | Number of molecules | 104 |
| Total formula weight | 7686.9 | |
| All* | Total formula weight | 473675.8 |
