3E1F

E.Coli (lacZ) beta-galactosidase (H418E) in complex with galactose

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000287	molecular_function	magnesium ion binding
1	0003824	molecular_function	catalytic activity
1	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
1	0004565	molecular_function	beta-galactosidase activity
1	0005975	biological_process	carbohydrate metabolic process
1	0005990	biological_process	lactose catabolic process
1	0009341	cellular_component	beta-galactosidase complex
1	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
1	0030246	molecular_function	carbohydrate binding
1	0031420	molecular_function	alkali metal ion binding
1	0042802	molecular_function	identical protein binding
1	0046872	molecular_function	metal ion binding
2	0000287	molecular_function	magnesium ion binding
2	0003824	molecular_function	catalytic activity
2	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
2	0004565	molecular_function	beta-galactosidase activity
2	0005975	biological_process	carbohydrate metabolic process
2	0005990	biological_process	lactose catabolic process
2	0009341	cellular_component	beta-galactosidase complex
2	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
2	0030246	molecular_function	carbohydrate binding
2	0031420	molecular_function	alkali metal ion binding
2	0042802	molecular_function	identical protein binding
2	0046872	molecular_function	metal ion binding
3	0000287	molecular_function	magnesium ion binding
3	0003824	molecular_function	catalytic activity
3	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
3	0004565	molecular_function	beta-galactosidase activity
3	0005975	biological_process	carbohydrate metabolic process
3	0005990	biological_process	lactose catabolic process
3	0009341	cellular_component	beta-galactosidase complex
3	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
3	0030246	molecular_function	carbohydrate binding
3	0031420	molecular_function	alkali metal ion binding
3	0042802	molecular_function	identical protein binding
3	0046872	molecular_function	metal ion binding
4	0000287	molecular_function	magnesium ion binding
4	0003824	molecular_function	catalytic activity
4	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
4	0004565	molecular_function	beta-galactosidase activity
4	0005975	biological_process	carbohydrate metabolic process
4	0005990	biological_process	lactose catabolic process
4	0009341	cellular_component	beta-galactosidase complex
4	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
4	0030246	molecular_function	carbohydrate binding
4	0031420	molecular_function	alkali metal ion binding
4	0042802	molecular_function	identical protein binding
4	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00608
Number of Residues	15
Details	GLYCOSYL_HYDROL_F2_2 Glycosyl hydrolases family 2 acid/base catalyst. DRNHPSVIIWSlg.NE

Chain	Residue	Details
1	ASP447-GLU461

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site_id	PS00719
Number of Residues	26
Details	GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV

Chain	Residue	Details
1	ASN385-VAL410

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:6420154

Chain	Residue	Details
1	GLU461
2	GLU461
3	GLU461
4	GLU461

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:1350782

Chain	Residue	Details
1	GLU537
2	GLU537
3	GLU537
4	GLU537

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site_id	SWS_FT_FI3
Number of Residues	28
Details	BINDING:

Chain	Residue	Details
2	ASN102
2	ASP201
2	GLU461
2	GLU537
2	PHE601
2	ASN604
2	TRP999
3	ASN102
3	ASP201
3	GLU461
3	GLU537
3	PHE601
3	ASN604
3	TRP999
4	ASN102
4	ASP201
4	GLU461
4	GLU537
4	PHE601
4	ASN604
4	TRP999
1	ASN102
1	ASP201
1	GLU461
1	GLU537
1	PHE601
1	ASN604
1	TRP999

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:11045615

Chain	Residue	Details
1	GLU416
1	GLU418
2	GLU416
2	GLU418
3	GLU416
3	GLU418
4	GLU416
4	GLU418
1	ASN597
2	ASN597
3	ASN597
4	ASN597

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	SITE: Transition state stabilizer

Chain	Residue	Details
3	HIS357
3	HIS391
4	HIS357
4	HIS391
1	HIS357
1	HIS391
2	HIS357
2	HIS391

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site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Important for ensuring that an appropriate proportion of lactose is converted to allolactose

Chain	Residue	Details
1	TRP999
2	TRP999
3	TRP999
4	TRP999

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
1	HIS357
1	HIS391
1	GLU416
1	GLU418
1	GLU461
1	GLU537
1	ASN597
1	PHE601
1	ASN604
1	ASP201

---

site_id	MCSA2
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
2	ASP201
2	HIS357
2	HIS391
2	GLU416
2	GLU418
2	GLU461
2	GLU537
2	ASN597
2	PHE601
2	ASN604

---

site_id	MCSA3
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
3	ASP201
3	HIS357
3	HIS391
3	GLU416
3	GLU418
3	GLU461
3	GLU537
3	ASN597
3	PHE601
3	ASN604

---

site_id	MCSA4
Number of Residues	10
Details	M-CSA 422

Chain	Residue	Details
4	ASP201
4	HIS357
4	HIS391
4	GLU416
4	GLU418
4	GLU461
4	GLU537
4	ASN597
4	PHE601
4	ASN604

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