3E1F

E.Coli (lacZ) beta-galactosidase (H418E) in complex with galactose

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000287	molecular_function	magnesium ion binding
1	0003824	molecular_function	catalytic activity
1	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
1	0004565	molecular_function	beta-galactosidase activity
1	0005975	biological_process	carbohydrate metabolic process
1	0005990	biological_process	lactose catabolic process
1	0009341	cellular_component	beta-galactosidase complex
1	0016787	molecular_function	hydrolase activity
1	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
1	0030246	molecular_function	carbohydrate binding
1	0031420	molecular_function	alkali metal ion binding
1	0042802	molecular_function	identical protein binding
1	0046872	molecular_function	metal ion binding
2	0000287	molecular_function	magnesium ion binding
2	0003824	molecular_function	catalytic activity
2	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
2	0004565	molecular_function	beta-galactosidase activity
2	0005975	biological_process	carbohydrate metabolic process
2	0005990	biological_process	lactose catabolic process
2	0009341	cellular_component	beta-galactosidase complex
2	0016787	molecular_function	hydrolase activity
2	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
2	0030246	molecular_function	carbohydrate binding
2	0031420	molecular_function	alkali metal ion binding
2	0042802	molecular_function	identical protein binding
2	0046872	molecular_function	metal ion binding
3	0000287	molecular_function	magnesium ion binding
3	0003824	molecular_function	catalytic activity
3	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
3	0004565	molecular_function	beta-galactosidase activity
3	0005975	biological_process	carbohydrate metabolic process
3	0005990	biological_process	lactose catabolic process
3	0009341	cellular_component	beta-galactosidase complex
3	0016787	molecular_function	hydrolase activity
3	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
3	0030246	molecular_function	carbohydrate binding
3	0031420	molecular_function	alkali metal ion binding
3	0042802	molecular_function	identical protein binding
3	0046872	molecular_function	metal ion binding
4	0000287	molecular_function	magnesium ion binding
4	0003824	molecular_function	catalytic activity
4	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
4	0004565	molecular_function	beta-galactosidase activity
4	0005975	biological_process	carbohydrate metabolic process
4	0005990	biological_process	lactose catabolic process
4	0009341	cellular_component	beta-galactosidase complex
4	0016787	molecular_function	hydrolase activity
4	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
4	0030246	molecular_function	carbohydrate binding
4	0031420	molecular_function	alkali metal ion binding
4	0042802	molecular_function	identical protein binding
4	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00608
Number of Residues	15
Details	GLYCOSYL_HYDROL_F2_2 Glycosyl hydrolases family 2 acid/base catalyst. DRNHPSVIIWSlg.NE

Chain	Residue	Details
1	ASP447-GLU461

---

site_id	PS00719
Number of Residues	26
Details	GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV

Chain	Residue	Details
1	ASN385-VAL410

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"6420154","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"1350782","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	36
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11045615","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Site: {"description":"Important for ensuring that an appropriate proportion of lactose is converted to allolactose"}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
1	GLU461
1	GLU537

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
2	GLU461
2	GLU537

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
3	GLU461
3	GLU537

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
4	GLU461
4	GLU537

---

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
1	TYR503
1	GLU461
1	GLU537

---

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
2	TYR503
2	GLU461
2	GLU537

---

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
3	TYR503
3	GLU461
3	GLU537

---

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1bgl

Chain	Residue	Details
4	TYR503
4	GLU461
4	GLU537

---

site_id	MCSA1
Number of Residues	10
Details	M-CSA 422

Chain

Residue

Details

---

site_id	MCSA2
Number of Residues	10
Details	M-CSA 422

Chain

Residue

Details

---

site_id	MCSA3
Number of Residues	10
Details	M-CSA 422

Chain

Residue

Details

---

site_id	MCSA4
Number of Residues	10
Details	M-CSA 422

Chain

Residue

Details

---