3E0D

Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, E	DNA substrate template strand	polymer	27	8287.3	2
2	D, F	DNA substrate primer strand	polymer	21	6411.2	2
3	A, B	DNA polymerase III subunit alpha	polymer	1220	137614.4	2	UniProt (Q9XDH5) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) Pfam (PF01336) In PDB	Thermus aquaticus
4	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	A, B	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	2	Chemie (DTP)

Sequence modifications

A, B: 1 - 1220 (UniProt: Q9XDH5)

PDB	External Database	Details
Asn 20	Asp 20	engineered mutation
Asn 212	Asp 212	engineered mutation
Phe 539	Ile 539	engineered mutation
Glu 540	Gln 540	engineered mutation
Ala 541	Val 541	engineered mutation
Glu 542	Val 542	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	304625.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1062.5
All*	Total formula weight	305688.2

*Water molecules are not included.