3E0D
Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (C, E) | DNA substrate template strand | polymer | 27 | 8287.3 | 2 | |||
| 2 | B, D (D, F) | DNA substrate primer strand | polymer | 21 | 6411.2 | 2 | |||
| 3 | E, F (A, B) | DNA polymerase III subunit alpha | polymer | 1220 | 137614.4 | 2 | UniProt (Q9XDH5) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) | Thermus aquaticus | |
| 4 | G, I (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | H, J (A, B) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (DTP) |
Sequence modifications
A, B: 1 - 1220 (UniProt: Q9XDH5)
| PDB | External Database | Details |
|---|---|---|
| Asn 20 | Asp 20 | engineered mutation |
| Asn 212 | Asp 212 | engineered mutation |
| Phe 539 | Ile 539 | engineered mutation |
| Glu 540 | Gln 540 | engineered mutation |
| Ala 541 | Val 541 | engineered mutation |
| Glu 542 | Val 542 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 304625.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1062.5 | |
| All* | Total formula weight | 305688.2 |
