3E0D
Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-03-09 |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 |
Unit cell lengths | 149.546, 149.546, 163.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.600 |
Rwork | 0.287 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 4.600 | 4.600 |
Number of reflections | 20097 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 16 - 21% PEG 400, 100 mM CaCl2, 100 mM HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K | |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 16 - 21% PEG 400, 100 mM CaCl2, 100 mM HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 400 | ||
6 | 1 | 2 | CaCl2 | ||
7 | 1 | 2 | HEPES | ||
8 | 1 | 2 | H2O |