3DRW

Crystal Structure of a Phosphofructokinase from Pyrococcus horikoshii OT3 with AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ADP-specific phosphofructokinase	polymer	474	54495.3	2	UniProt (O59355) Pfam (PF04587) In PDB	Pyrococcus horikoshii	ADP-dependent phosphofructokinase, ADP-Pfk
2	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4		water	water		18.0	576	Chemie (HOH)

Sequence modifications

A, B: 1 - 450 (UniProt: O59355)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Arg -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 17	Asp 17	engineered mutation
Gly 451	-	expression tag
Ser 452	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	108990.7
Non-Polymers*	Number of molecules	4
	Total formula weight	740.4
All*	Total formula weight	109731.1

*Water molecules are not included.