3DQV

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	NEDD8	polymer	81	9078.0	2	UniProt (Q15843) Pfam (PF00240)	Homo sapiens (human)	Ubiquitin-like protein Nedd8, Neddylin
2	B, E (C, D)	Cullin-5	polymer	382	45601.8	2	UniProt (Q93034) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (human)	CUL-5, Vasopressin-activated calcium-mobilizing receptor, VACM-1
3	C, F (R, Y)	Rbx1	polymer	106	12183.5	2	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	Rbx1, Regulator of cullins 1, RING finger protein 75, Protein ZYP
4	G, H, I, J, K... (R, Y)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, B: 101 - 176 (UniProt: Q15843)

PDB	External Database	Details
Gly 96	-	insertion
Ser 97	-	insertion
Gly 98	-	insertion
Gly 99	-	insertion
Ser 100	-	insertion
Mse 162	Leu 62	conflict

C, D: 1401 - 1780 (UniProt: Q93034)

PDB	External Database	Details
Gly 1399	-	insertion
Ser 1400	-	insertion
Glu 1407	Leu 407	conflict
Lys 1439	Leu 439	conflict
Lys 1440	Val 440	conflict

R, Y: 5 - 108 (UniProt: P62877)

PDB	External Database	Details
Gly 3	-	insertion
Ser 4	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	133726.6
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	134119.1

*Water molecules are not included.