3DPC
Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Alkaline phosphatase | polymer | 455 | 47949.3 | 2 | UniProt (P00634) Pfam (PF00245) In PDB | Escherichia coli | APase |
| 2 | C | Phosphorylated Peptide | polymer | 10 | 1176.2 | 1 | PDB (3DPC) | ||
| 3 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 4 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | water | water | 18.0 | 221 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 449 (UniProt: P00634)
| PDB | External Database | Details |
|---|---|---|
| Leu 102 | Ser 124 | engineered mutation |
| His 450 | - | expression tag |
| His 451 | - | expression tag |
| His 452 | - | expression tag |
| His 453 | - | expression tag |
| His 454 | - | expression tag |
| His 455 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 97074.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 217.1 | |
| All* | Total formula weight | 97292.0 |
