3DPC
Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.862, 107.740, 148.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.234 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.598 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.272 |
| Number of reflections | 46934 | |
| <I/σ(I)> | 8 | 3.5 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 7.51 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 314 | 39%-43% saturation ammonium sulfate, 100mM Tris pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 314K |
