3DKH

L559A mutant of Melanocarpus albomyces laccase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Laccase-1	polymer	559	61803.4	2	UniProt (Q70KY3) Pfam (PF07732) Pfam (PF00394) Pfam (PF07731)	Melanocarpus albomyces	Benzenediol:oxygen oxidoreductase 1, Urishiol oxidase 1, Diphenol oxidase 1, Ligninolytic phenoloxidase
2	C (C)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
3	D, F, G, K (D, F, G, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	E, H, I, J (E, H, I, J)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
5	L, M, V, W, X... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
6	AA, BA, CA, N, O... (B, A)	COPPER (II) ION	non-polymer		63.5	8	Chemie (CU)
7	R (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	DA, S, T (B, A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
9	EA, U (B, A)	OXYGEN MOLECULE	non-polymer		32.0	2	Chemie (OXY)
10	FA (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
11	GA, HA (A, B)	water	water		18.0	706	Chemie (HOH)

Sequence modifications

A, B: 1 - 559 (UniProt: Q70KY3)

PDB	External Database	Details
Ala 559	Leu 609	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	123606.7
Branched	Number of molecules	9
	Total formula weight	4792.5
Non-Polymers*	Number of molecules	21
	Total formula weight	2315.3
All*	Total formula weight	130714.5

*Water molecules are not included.