3DKH
L559A mutant of Melanocarpus albomyces laccase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.365 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.510, 62.020, 125.670 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.400 |
| Rwork | 0.224 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q9o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 50625 | 5497 |
| <I/σ(I)> | 9.05 | 4 |
| Completeness [%] | 97.4 | 92.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 15% PEG MME 2000, 0.2M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
