3DKD
Crystal structure of the mimivirus NDK +Kpn-N62L-R107G triple mutant complexed with GDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nucleoside diphosphate kinase | polymer | 146 | 16527.7 | 2 | UniProt (Q5UQL3) Pfam (PF00334) | Acanthamoeba polyphaga mimivirus (APMV) | NDP kinase, NDK |
| 2 | C, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D, F (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 248 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 141 (UniProt: Q5UQL3)
| PDB | External Database | Details |
|---|---|---|
| Tyr -4 | - | expression tag |
| Lys -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Leu 62 | Asn 62 | engineered mutation |
| Thr 92 | - | SEE REMARK 999 |
| Asn 93 | - | SEE REMARK 999 |
| Pro 94 | - | SEE REMARK 999 |
| Leu 95 | - | SEE REMARK 999 |
| Ala 96 | Ile 92 | SEE REMARK 999 |
| Ser 97 | Leu 93 | SEE REMARK 999 |
| Ala 98 | Thr 94 | SEE REMARK 999 |
| Gly 111 | Arg 107 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 33055.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 935.0 | |
| All* | Total formula weight | 33990.4 |
