3D9O

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RNA-binding protein 16	polymer	145	17160.2	2	UniProt (Q9UPN6) Pfam (PF04818) In PDB	Homo sapiens (man)	RNA-binding motif protein 16
2	Z	CTD-PEPTIDE	polymer	14	1457.5	1	Pfam (PF05001) UniProt (by SIFTS) (P24928) In PDB
3	A, B	AMMONIUM ION	non-polymer		18.0	2	Chemie (NH4)
4	A, B, Z	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
5		water	water		18.0	127	Chemie (HOH)

Sequence modifications

A, B: 1 - 136 (UniProt: Q9UPN6)

PDB	External Database	Details
Mse 15	Asn 15	engineered mutation
Mse 17	Tyr 17	engineered mutation
Ala 137	-	expression tag
Leu 138	-	expression tag
Glu 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	35778.0
Non-Polymers*	Number of molecules	8
	Total formula weight	612.5
All*	Total formula weight	36390.4

*Water molecules are not included.