3D3B

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N utilization substance protein B	polymer	141	15838.1	1	UniProt (P0A780) Pfam (PF01029)	Escherichia coli	Protein nusB
2	B (J)	30S ribosomal protein S10	polymer	87	9608.1	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli
3	C, D, E (A, J)	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	3	Chemie (NHE)
4	F, G (A, J)	water	water		18.0	316	Chemie (HOH)

Sequence modifications

A: 1 - 139 (UniProt: P0A780)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Glu 2	Lys 2	engineered mutation

J: 1 - 45 (UniProt: P0A7R5)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

J: 47 - 82 (UniProt: P0A7R5)

PDB	External Database	Details
Ser 46	-	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25446.2
Non-Polymers*	Number of molecules	3
	Total formula weight	621.9
All*	Total formula weight	26068.1

*Water molecules are not included.