3D3B
Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9051 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.740, 48.990, 112.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.17504 |
| Rwork | 0.173 |
| R-free | 0.20448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1TZV and PDB ID 1J5E (chain J) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.512 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.074 | 0.648 |
| Number of reflections | 56411 | |
| <I/σ(I)> | 17.5 | 5.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.3 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 0.1 M CHES, pH 8.8, 18 % PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
