3CX4
Crystal Structure of E.coli GS mutant E377A in complex with ADP and oligosaccharides
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glycogen synthase | polymer | 485 | 53893.3 | 1 | UniProt (P0A6U8) Pfam (PF08323) Pfam (PF00534) | Escherichia coli | Starch [bacterial glycogen] synthase |
| 2 | B (B) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900009) GlyTouCan (G96370VA) | alpha-maltotriose | ||
| 3 | C (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | D (D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 1 | In PDB BIRD (PRD_900030) GlyTouCan (G50146AM) | alpha-maltopentaose | ||
| 5 | E (E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 990.9 | 1 | In PDB GlyTouCan (G86211GT) | |||
| 6 | F, G (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 7 | H (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 8 | I (A) | (2R)-2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid | non-polymer | 268.3 | 1 | Chemie (250) | |||
| 9 | J, K, L, M, N... (A) | 2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL | non-polymer | 208.3 | 12 | Chemie (ETE) | |||
| 10 | V (A) | water | water | 18.0 | 201 | Chemie (HOH) |
Sequence modifications
A: 1 - 477 (UniProt: P0A6U8)
| PDB | External Database | Details |
|---|---|---|
| Ala 377 | Glu 377 | engineered mutation |
| Leu 478 | - | expression tag |
| Glu 479 | - | expression tag |
| His 480 | - | expression tag |
| His 481 | - | expression tag |
| His 482 | - | expression tag |
| His 483 | - | expression tag |
| His 484 | - | expression tag |
| His 485 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 53893.3 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2666.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 3240.5 | |
| All* | Total formula weight | 59800.2 |
