3CX4
Crystal Structure of E.coli GS mutant E377A in complex with ADP and oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 126.324, 126.324, 152.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.310 - 2.290 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qzs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.433 |
| Data reduction software | X-GEN |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.085 | 0.530 |
| Number of reflections | 53700 | |
| <I/σ(I)> | 14.5 | 1.06 |
| Completeness [%] | 98.1 | 99 |
| Redundancy | 3.21 | 2.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40 % (w/v) PEG 4000, 0.1 M HEPPSO, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
