3CUS
Structure of a double ILE/PHE mutant of NI-FE hydrogenase refined at 2.2 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (A, B, C) | Periplasmic [NiFe] hydrogenase small subunit | polymer | 264 | 28347.3 | 3 | UniProt (P18187) | Desulfovibrio fructosovorans | NiFe hydrogenlyase small chain |
| 2 | B, D, F (Q, R, S) | Periplasmic [NiFe] hydrogenase large subunit | polymer | 549 | 59836.4 | 3 | UniProt (P18188) | Desulfovibrio fructosovorans | NiFe hydrogenlyase large chain |
| 3 | G, I, P, R, W... (A, B, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 4 | H, Q, X (A, B, C) | FE3-S4 CLUSTER | non-polymer | 295.8 | 3 | Chemie (F3S) | |||
| 5 | CA, J, N, O, V (S, A, Q, R) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | K, S, Z (Q, R, S) | NICKEL (II) ION | non-polymer | 58.7 | 3 | Chemie (NI) | |||
| 7 | AA, L, T (S, Q, R) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 8 | BA, M, U (S, Q, R) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 3 | Chemie (FCO) | |||
| 9 | DA, EA, FA, GA, HA... (A, Q, B, R, C...) | water | water | 18.0 | 1013 | Chemie (HOH) |
Sequence modifications
Q, R, S: 1 - 549 (UniProt: P18188)
| PDB | External Database | Details |
|---|---|---|
| Ile 74 | Val 74 | engineered mutation |
| Phe 122 | Leu 122 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 264551.1 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 4114.4 | |
| All* | Total formula weight | 268665.4 |
