Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3CUS

Structure of a double ILE/PHE mutant of NI-FE hydrogenase refined at 2.2 angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008901	molecular_function	ferredoxin hydrogenase activity
A	0009055	molecular_function	electron transfer activity
A	0009061	biological_process	anaerobic respiration
A	0009375	cellular_component	ferredoxin hydrogenase complex
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0042597	cellular_component	periplasmic space
A	0044569	cellular_component	[Ni-Fe] hydrogenase complex
A	0046872	molecular_function	metal ion binding
A	0047806	molecular_function	cytochrome-c3 hydrogenase activity
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051538	molecular_function	3 iron, 4 sulfur cluster binding
A	0051539	molecular_function	4 iron, 4 sulfur cluster binding
B	0008901	molecular_function	ferredoxin hydrogenase activity
B	0009055	molecular_function	electron transfer activity
B	0009061	biological_process	anaerobic respiration
B	0009375	cellular_component	ferredoxin hydrogenase complex
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0042597	cellular_component	periplasmic space
B	0044569	cellular_component	[Ni-Fe] hydrogenase complex
B	0046872	molecular_function	metal ion binding
B	0047806	molecular_function	cytochrome-c3 hydrogenase activity
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051538	molecular_function	3 iron, 4 sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0008901	molecular_function	ferredoxin hydrogenase activity
C	0009055	molecular_function	electron transfer activity
C	0009061	biological_process	anaerobic respiration
C	0009375	cellular_component	ferredoxin hydrogenase complex
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0042597	cellular_component	periplasmic space
C	0044569	cellular_component	[Ni-Fe] hydrogenase complex
C	0046872	molecular_function	metal ion binding
C	0047806	molecular_function	cytochrome-c3 hydrogenase activity
C	0051536	molecular_function	iron-sulfur cluster binding
C	0051538	molecular_function	3 iron, 4 sulfur cluster binding
C	0051539	molecular_function	4 iron, 4 sulfur cluster binding
Q	0008901	molecular_function	ferredoxin hydrogenase activity
Q	0016151	molecular_function	nickel cation binding
Q	0016491	molecular_function	oxidoreductase activity
Q	0042597	cellular_component	periplasmic space
Q	0046872	molecular_function	metal ion binding
Q	0047806	molecular_function	cytochrome-c3 hydrogenase activity
R	0008901	molecular_function	ferredoxin hydrogenase activity
R	0016151	molecular_function	nickel cation binding
R	0016491	molecular_function	oxidoreductase activity
R	0042597	cellular_component	periplasmic space
R	0046872	molecular_function	metal ion binding
R	0047806	molecular_function	cytochrome-c3 hydrogenase activity
S	0008901	molecular_function	ferredoxin hydrogenase activity
S	0016151	molecular_function	nickel cation binding
S	0016491	molecular_function	oxidoreductase activity
S	0042597	cellular_component	periplasmic space
S	0046872	molecular_function	metal ion binding
S	0047806	molecular_function	cytochrome-c3 hydrogenase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NI Q 551

Chain	Residue
Q	CYS72
Q	CYS75
Q	CYS546

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG Q 553

Chain	Residue
Q	GLU53
Q	LEU495
Q	HIS549
Q	HOH563
Q	HOH564
Q	HOH565

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NI R 551

Chain	Residue
R	CYS72
R	CYS75
R	CYS546

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG R 553

Chain	Residue
R	GLU53
R	LEU495
R	HIS549
R	HOH564
R	HOH565
R	HOH566

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NI S 551

Chain	Residue
S	CYS72
S	CYS75
S	CYS546

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG S 553

Chain	Residue
S	GLU53
S	LEU495
S	HIS549
S	HOH562
S	HOH563
S	HOH564

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SF4 A 265

Chain	Residue
A	HIS184
A	CYS187
A	ARG189
A	LEU190
A	CYS212
A	LEU213
A	CYS218

site_id	AC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE F3S A 266

Chain	Residue
A	ASN225
A	CYS227
A	PHE232
A	TRP237
A	CYS245
A	LEU246
A	CYS248
Q	GLN230

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SF4 A 267

Chain	Residue
A	GLU16
A	CYS17
A	CYS20
A	THR113
A	CYS114
A	GLY146
A	CYS147
A	PRO148
Q	ARG70
Q	HIS228

site_id	BC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FCO Q 550

Chain	Residue
Q	CYS75
Q	HIS79
Q	ALA474
Q	PRO475
Q	ARG476
Q	LEU479
Q	PRO498
Q	SER499
Q	CYS546

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SF4 B 265

Chain	Residue
B	HIS184
B	CYS187
B	ARG189
B	LEU190
B	CYS212
B	LEU213
B	TYR214
B	CYS218

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE F3S B 266

Chain	Residue
B	ASN225
B	CYS227
B	TRP237
B	CYS245
B	LEU246
B	CYS248
R	LYS225
R	GLN230

site_id	BC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SF4 B 267

Chain	Residue
B	GLU16
B	CYS17
B	CYS20
B	THR113
B	CYS114
B	GLY146
B	CYS147
B	PRO148
R	ARG70
R	HIS228

site_id	BC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FCO R 550

Chain	Residue
R	CYS75
R	HIS79
R	ALA474
R	PRO475
R	ARG476
R	LEU479
R	PRO498
R	SER499
R	CYS546

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SF4 C 265

Chain	Residue
C	LEU190
C	CYS212
C	LEU213
C	CYS218
C	HIS184
C	CYS187
C	ARG189

site_id	BC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE F3S C 266

Chain	Residue
C	ASN225
C	CYS227
C	PHE232
C	TRP237
C	CYS245
C	LEU246
C	CYS248
S	GLN230

site_id	BC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SF4 C 267

Chain	Residue
C	GLU16
C	CYS17
C	CYS20
C	THR113
C	CYS114
C	GLY146
C	CYS147
C	PRO148
S	ARG70
S	HIS228

site_id	BC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FCO S 550

Chain	Residue
S	CYS75
S	HIS79
S	ALA474
S	PRO475
S	ARG476
S	LEU479
S	PRO498
S	SER499
S	CYS546

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 271

Chain	Residue
A	ASP168
A	LEU169
A	LYS176
A	HOH306

site_id	CC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL Q 561

Chain	Residue
Q	ARG100
Q	ASN104
Q	PHE295
Q	ALA296
Q	THR297
Q	GLU445
Q	HOH587
Q	HOH767

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL Q 562

Chain	Residue
A	ALA55
C	PHE198
Q	ASN181
Q	ALA182
Q	LEU185
Q	ARG529
Q	HOH567

site_id	CC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL R 563

Chain	Residue
R	ARG100
R	ASN104
R	PHE295
R	ALA296
R	THR297
R	GLN310
R	TRP442
R	GLU445

site_id	CC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL S 561

Chain	Residue
S	ARG100
S	ASN104
S	PHE295
S	ALA296
S	THR297
S	GLU445
S	HOH583
S	HOH724

Functional Information from PROSITE/UniProt

site_id	PS00507
Number of Residues	26
Details	NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilkgrdprdaqhftQRaCGIC

Chain	Residue	Details
Q	ARG50-CYS75

site_id	PS00508
Number of Residues	10
Details	NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCIACgv.H

Chain	Residue	Details
Q	PHE540-HIS549

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	33
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
Q	GLU25

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
R	GLU25

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
S	GLU25

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
A	THR18

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
B	THR18

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1cc1

Chain	Residue	Details
C	THR18

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)