3CPT

MP1-p14 Scaffolding complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mitogen-activated protein kinase kinase 1-interacting protein 1	polymer	143	15913.0	1	UniProt (Q9UHA4) Pfam (PF08923) In PDB	Homo sapiens (Human)	MEK-binding partner 1, Mp1
2	B	Mitogen-activated protein-binding protein-interacting protein	polymer	131	14227.2	1	UniProt (Q9JHS3) Pfam (PF03259) In PDB	Mus musculus (Mouse)	Late endosomal/lysosomal Mp1-interacting protein, p14
3		water	water		18.0	154	Chemie (HOH)

Sequence modifications

A: 2 - 124 (UniProt: Q9UHA4)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

B: 2 - 125 (UniProt: Q9JHS3)

PDB	External Database	Details
Met -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ala 56	Tyr 56	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30140.3
All*	Total formula weight	30140.3

*Water molecules are not included.