3CPT

MP1-p14 Scaffolding complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 21 21 21
Unit cell lengths	44.950, 63.140, 75.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 1.900
R-factor	0.20454
Rwork	0.201
R-free	0.27718
RMSD bond length	0.011
RMSD bond angle	1.265
Data scaling software	d*TREK (9.5L)
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.510	26.330	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.056	0.036	0.288
Total number of observations		8829	8831
Number of reflections	17610
<I/σ(I)>	13.4	29.3	4.5
Completeness [%]	100.0	100	100
Redundancy	5	4.64	5.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	10%(w/v) PEG 3350, 0.1 M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 295K