3CMJ

Crystal Structure of engineered Beta-Glucosidase from Soil metagenome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-glucosidase	polymer	465	52596.8	1	UniProt (Q0GMU3) Pfam (PF00232) In PDB	Uncultured bacterium
2	A	SODIUM ION	non-polymer		23.0	22	Chemie (NA)
3	A	S,R MESO-TARTARIC ACID	non-polymer		150.1	1	Chemie (SRT)
4		water	water		18.0	367	Chemie (HOH)

Sequence modifications

A: 39 - 482 (UniProt: Q0GMU3)

PDB	External Database	Details
Met 18	-	expression tag
Gly 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Ser 28	-	expression tag
Ser 29	-	expression tag
Gly 30	-	expression tag
Val 32	-	expression tag
Arg 34	-	expression tag
Gly 35	-	expression tag
Ser 36	-	expression tag
His 37	-	expression tag
Met 38	-	expression tag
Asn 39	Glu 39	engineered mutation
Val 40	Leu 40	engineered mutation
Lys 41	Gln 41	engineered mutation
Lys 42	Pro 42	engineered mutation
Glu 45	Lys 45	engineered mutation
Asn 477	Arg 477	engineered mutation
Glu 481	Ala 481	engineered mutation
Asp 482	Ala 482	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52596.8
Non-Polymers*	Number of molecules	23
	Total formula weight	655.9
All*	Total formula weight	53252.7

*Water molecules are not included.