3C5G
Structure of a ternary complex of the R517K Pol lambda mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, B) | DNA polymerase lambda | polymer | 335 | 37451.7 | 2 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Lambda, DNA polymerase kappa, DNA polymerase beta-2, Pol beta2 |
| 2 | B, F (T, U) | DNA (5'-D(*DCP*DGP*DGP*DCP*DAP*DAP*DTP*DAP*DCP*DTP*DG)-3') | polymer | 11 | 3358.2 | 2 | |||
| 3 | C, G (P, Q) | DNA (5'-D(*DCP*DAP*DGP*DTP*DAP*(2DT))-3') | polymer | 6 | 1792.2 | 2 | |||
| 4 | D, H (D, E) | DNA (5'-D(P*DGP*DCP*DCP*DG)-3') | polymer | 4 | 1191.8 | 2 | |||
| 5 | I, Q (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | J, K, L, O, R... (A, P, B) | SODIUM ION | non-polymer | 23.0 | 8 | Chemie (NA) | |||
| 7 | M, P (A, B) | 2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 466.2 | 2 | Chemie (D3T) | |||
| 8 | N (T) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 9 | AA, BA, CA, V, W... (U, Q, E, A, T...) | water | water | 18.0 | 838 | Chemie (HOH) |
Sequence modifications
A, B: 242 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Met 241 | - | expression tag |
| Lys 517 | Arg 517 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 87588.0 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1226.9 | |
| All* | Total formula weight | 88814.9 |
