3BXM

Structure of an inactive mutant of human glutamate carboxypeptidase II [GCPII(E424A)] in complex with N-acetyl-Asp-Glu (NAAG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glutamate carboxypeptidase 2	polymer	709	79801.0	1	UniProt (Q04609) Pfam (PF02225) Pfam (PF04389) Pfam (PF04253) In PDB	Homo sapiens (human)	Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Folate hydrolase 1, Prostate-specific membrane antigen, PSMA, PSM
2	I	N-Acetyl-Aspartyl-Glutamate (NAAG)	polymer	3	288.3	1
3	B, C, D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	E	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
6	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9		water	water		18.0	496	Chemie (HOH)

Sequence modifications

A: 44 - 750 (UniProt: Q04609)

PDB	External Database	Details
Arg 42	-	expression tag
Ser 43	-	expression tag
Ala 424	Glu 424	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80089.2
Branched	Number of molecules	4
	Total formula weight	2021.9
Non-Polymers*	Number of molecules	7
	Total formula weight	870.0
All*	Total formula weight	82981.1

*Water molecules are not included.