3BSH
Barley alpha-amylase isozyme 1 (AMY1) double mutant Y105A/Y380A in complex with inhibitor acarbose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha-amylase type A isozyme | polymer | 414 | 45312.8 | 1 | UniProt (P00693) Pfam (PF00128) Pfam (PF07821) | Hordeum vulgare (Barley) | alpha-amylase isozyme 1, 1,4-alpha-D- glucan glucanohydrolase, AMY1, Low pI alpha-amylase |
| 2 | B (A) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 3 | C, D, E (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 4 | F (A) | water | water | 18.0 | 112 | Chemie (HOH) |
Sequence modifications
A: 1 - 414 (UniProt: P00693)
| PDB | External Database | Details |
|---|---|---|
| Ala 105 | Tyr 129 | engineered mutation |
| Val 284 | Ala 308 | SEE REMARK 999 |
| Ala 380 | Tyr 404 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 45312.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 300.4 | |
| All* | Total formula weight | 45613.2 |
