3BF4

Crystal structure of an ethd-like protein (reut_b5694) from ralstonia eutropha jmp134 at 2.10 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ethyl tert-butyl ether degradation EthD protein	polymer	127	14546.4	2	UniProt (Q46P95) Pfam (PF07110) In PDB	Ralstonia eutropha JMP134
2	A, B	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
3	B	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
4		water	water		18.0	183	Chemie (HOH)

Sequence modifications

A, B: 1 - 108 (UniProt: Q46P95)

PDB	External Database	Details
Mse -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Asp -15	-	expression tag
Lys -14	-	expression tag
Ile -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Tyr 72	Gln 72	engineered mutation
Tyr 76	Gln 76	engineered mutation
Tyr 77	Glu 77	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29092.8
Non-Polymers*	Number of molecules	6
	Total formula weight	370.4
All*	Total formula weight	29463.2

*Water molecules are not included.