3BEU
Na+-Dependent Allostery Mediates Coagulation Factor Protease Active Site Selectivity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Trypsin | polymer | 224 | 23539.5 | 2 | UniProt (P00775) Pfam (PF00089) | Streptomyces griseus | SGT |
| 2 | C, G, H, I, L... (A, B) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 3 | D, J, K, O (A, B) | BENZAMIDINE | non-polymer | 120.2 | 4 | Chemie (BEN) | |||
| 4 | E, P (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | F, Q (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | V, W (A, B) | water | water | 18.0 | 605 | Chemie (HOH) |
Sequence modifications
A, B: 16 - 245 (UniProt: P00775)
| PDB | External Database | Details |
|---|---|---|
| Phe 94 | Tyr 112 | engineered mutation |
| Thr 95 | Asn 113 | engineered mutation |
| Lys 96 | Gly 114 | engineered mutation |
| Glu 97 | Thr 114 | engineered mutation |
| Thr 98 | - | SEE REMARK 999 |
| Tyr 99 | - | SEE REMARK 999 |
| Ser 171 | Ala 180 | engineered mutation |
| Ser 172 | Tyr 181 | engineered mutation |
| Ser 173 | Gly 182 | engineered mutation |
| Phe 174 | Asn 183 | engineered mutation |
| Ile 175 | Glu 184 | engineered mutation |
| Met 180 | Glu 190 | engineered mutation |
| - | Pro 196 | SEE REMARK 999 |
| Lys 186 | Gly 199 | engineered mutation |
| Gln 187 | Gly 200 | engineered mutation |
| Glu 188 | Val 201 | engineered mutation |
| Val 208 | Ile 222 | engineered mutation |
| Glu 217 | Tyr 231 | engineered mutation |
| Lys 223 | Pro 236 | engineered mutation |
| Lys 224 | Tyr 238 | engineered mutation |
| Tyr 225 | Pro 239 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 47078.9 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 1663.4 | |
| All* | Total formula weight | 48742.3 |
