3B07

Crystal structure of octameric pore form of gamma-hemolysin from Staphylococcus aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, C, E, G)	Gamma-hemolysin component B	polymer	309	35256.1	4	UniProt (Q931F3) Pfam (PF07968) UniProt (by SIFTS) (A0A0H3JX61)	Staphylococcus aureus	protomer F, H-gamma-1, H-gamma-I
2	E, F, G, H (B, D, F, H)	Gamma-hemolysin component A	polymer	290	33186.4	4	UniProt (P0A071) Pfam (PF07968)	Staphylococcus aureus	protomer S, H-gamma-2, H-gamma-II
3	I, J, K, L (A, C, E, G)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	4	Chemie (MPD)
4	M, N, O, P, Q... (A, C, E, G, B...)	water	water		18.0	449	Chemie (HOH)

Sequence modifications

A, C, E, G: 2 - 300 (UniProt: Q931F3)

PDB	External Database	Details
Met -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag

B, D, F, H: 1 - 280 (UniProt: P0A071)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	273769.7
Non-Polymers*	Number of molecules	4
	Total formula weight	472.7
All*	Total formula weight	274242.4

*Water molecules are not included.