3B07
Crystal structure of octameric pore form of gamma-hemolysin from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 206.454, 206.141, 190.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.298 - 2.495 |
| Rwork | 0.207 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1LKF 2QK7 and 3ANZ |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_617) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.298 | 44.000 | 2.650 |
| High resolution limit [Å] | 2.490 | 7.390 | 2.490 |
| Rmerge | 0.111 | 0.042 | 0.651 |
| Rmeas | 0.045 | 0.703 | |
| Number of reflections | 138838 | 5624 | 21660 |
| <I/σ(I)> | 14.32 | 32.54 | 3.47 |
| Completeness [%] | 98.9 | 98.9 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Sodium acetate, ammonium acetate, MPD, pH 4.6, vapor diffusion, sitting drop, temperature 293K |
