3B07
Crystal structure of octameric pore form of gamma-hemolysin from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-17 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 206.454, 206.141, 190.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.298 - 2.495 |
Rwork | 0.207 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1LKF 2QK7 and 3ANZ |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_617) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.298 | 44.000 | 2.650 |
High resolution limit [Å] | 2.490 | 7.390 | 2.490 |
Rmerge | 0.111 | 0.042 | 0.651 |
Rmeas | 0.045 | 0.703 | |
Number of reflections | 138838 | 5624 | 21660 |
<I/σ(I)> | 14.32 | 32.54 | 3.47 |
Completeness [%] | 98.9 | 98.9 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Sodium acetate, ammonium acetate, MPD, pH 4.6, vapor diffusion, sitting drop, temperature 293K |