3AY6

Crystal structure of Bacillus megaterium glucose dehydrogenase 4 A258F mutant in complex with NADH and D-glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glucose 1-dehydrogenase 4	polymer	269	29275.2	4	UniProt (P39485) Pfam (PF13561)	Bacillus megaterium	GLCDH-IV
2	E, I, K, N (A, B, C, D)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	4	Chemie (NAI)
3	F, H, L, M (A, B, C, D)	beta-D-glucopyranose	non-polymer		180.2	4	Chemie (BGC)
4	G, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	O, P, Q, R (A, B, C, D)	water	water		18.0	462	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 261 (UniProt: P39485)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 258	Ala 258	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117100.6
Non-Polymers*	Number of molecules	10
	Total formula weight	3453.3
All*	Total formula weight	120553.9

*Water molecules are not included.