3AY6
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 A258F mutant in complex with NADH and D-glucose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glucose 1-dehydrogenase 4 | polymer | 269 | 29275.2 | 4 | UniProt (P39485) | Bacillus megaterium | GLCDH-IV |
| 2 | E, I, K, N (A, B, C, D) | 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE | non-polymer | 665.4 | 4 | Chemie (NAI) PubChem (16219774) PubChem (44119956) PubChem (44415182) PubChem (439153) PubChem (124934832) PubChem (126455899) PubChem (134693949) PubChem (12309339) PubChem (12309347) PubChem (140875346) PubChem (162951601) PubChem (162951600) PubChem (164575491) PubChem (169545638) PubChem (170905224) PubChem (174316169) PubChem (94865921) PubChem (94865923) PubChem (928) PubChem (124432988) PubChem (124934831) PubChem (124934833) PubChem (11250888) PubChem (129607000) PubChem (129628338) PubChem (12309341) PubChem (146167236) PubChem (162951602) PubChem (174084875) | |||
| 3 | F, H, L, M (A, B, C, D) | beta-D-glucopyranose | non-polymer | 180.2 | 4 | Chemie (BGC) | |||
| 4 | G, J (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 5 | O, P, Q, R (A, B, C, D) | water | water | 18.0 | 462 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 261 (UniProt: P39485)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | expression tag |
| Gly -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Phe 258 | Ala 258 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 117100.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3453.3 | |
| All* | Total formula weight | 120553.9 |
