3AJV

Splicing endonuclease from Aeropyrum pernix

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Putative uncharacterized protein	polymer	190	20770.8	2	UniProt (Q9YE85) Pfam (PF22502) Pfam (PF01974)	Aeropyrum pernix	tRNA-splicing endonuclease subunit alpha
2	B, D (B, D)	tRNA-splicing endonuclease	polymer	186	20561.5	2	UniProt (Q9YBF1) Pfam (PF02778) Pfam (PF01974)	Aeropyrum pernix	tRNA-intron endonuclease
3	E, F, G, H, I... (A, B, C, D)	GLYCEROL	non-polymer		92.1	10	Chemie (GOL)
4	K, P (B, D)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	549	Chemie (HOH)

Sequence modifications

A, C: 1 - 170 (UniProt: Q9YE85)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B, D: 1 - 186 (UniProt: Q9YBF1)

PDB	External Database	Details
Ala 133	His 133	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82664.6
Non-Polymers*	Number of molecules	12
	Total formula weight	991.8
All*	Total formula weight	83656.5

*Water molecules are not included.