3AH8

Structure of heterotrimeric G protein Galpha-q beta gamma in complex with an inhibitor YM-254890

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1/Guanine nucleotide-binding protein G(q) subunit alpha chimeric protein	polymer	355	41427.0	1	UniProt (P10824) UniProt (P21279) Pfam (PF00503)	Rattus norvegicus (Rat Mouse)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	78	8592.9	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
4	D (Y)	YM-254890	polymer	9	962.1	1	BIRD (PRD_000482)	Chromobacterium sp.
5	E (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

A: 8 - 34 (UniProt: P10824)

PDB	External Database	Details
Gly 5	-	expression tag
Ala 6	-	expression tag
Met 7	-	expression tag
Arg 35	-	linker
Ser 36	-	linker

A: 37 - 359 (UniProt: P21279)
G: 8 - 78 (UniProt: P63212)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Met 3	-	expression tag
Asp 4	-	expression tag
Pro 5	-	expression tag
Glu 6	-	expression tag
Phe 7	-	expression tag
Ser 75	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88398.9
Non-Polymers*	Number of molecules	1
	Total formula weight	443.2
All*	Total formula weight	88842.1

*Water molecules are not included.