3AH8

Structure of heterotrimeric G protein Galpha-q beta gamma in complex with an inhibitor YM-254890

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1/Guanine nucleotide-binding protein G(q) subunit alpha chimeric protein	polymer	355	41427.0	1	UniProt (P10824) UniProt (P21279) Pfam (PF00503)	Rattus norvegicus (Rat Mouse)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF25391)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	78	8592.9	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
4	D (Y)	YM-254890	polymer	9	962.1	1	BIRD (PRD_000482)	Chromobacterium sp.
5	E (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)

Sequence modifications

A: 8 - 34 (UniProt: P10824)

PDB	External Database	Details
Gly 5	-	expression tag
Ala 6	-	expression tag
Met 7	-	expression tag
Arg 35	-	linker
Ser 36	-	linker

A: 37 - 359 (UniProt: P21279)
G: 8 - 78 (UniProt: P63212)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Met 3	-	expression tag
Asp 4	-	expression tag
Pro 5	-	expression tag
Glu 6	-	expression tag
Phe 7	-	expression tag
Ser 75	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88398.9
Non-Polymers*	Number of molecules	1
	Total formula weight	443.2
All*	Total formula weight	88842.1

*Water molecules are not included.