3ACT

Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cellobiose Phosphorylase	polymer	842	93047.8	2	UniProt (O66264) Pfam (PF06165) Pfam (PF17167)	Cellvibrio gilvus
2	C, G (A, B)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
3	D, H (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	E, I (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	F, J, K, L, M (A, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	N, O (A, B)	water	water		18.0	2202	Chemie (HOH)

Sequence modifications

A, B: 1 - 822 (UniProt: O66264)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 666	His 666	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	186095.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1088.9
All*	Total formula weight	187184.4

*Water molecules are not included.