3ACS

Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase W488F mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cellobiose Phosphorylase	polymer	842	93032.8	2	UniProt (O66264) Pfam (PF06165) Pfam (PF17167)	Cellvibrio gilvus
2	C, E (A, B)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
3	D, F (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	G, H (A, B)	water	water		18.0	826	Chemie (HOH)

Sequence modifications

A, B: 1 - 822 (UniProt: O66264)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Phe 488	Trp 488	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	186065.5
Non-Polymers*	Number of molecules	4
	Total formula weight	552.4
All*	Total formula weight	186618.0

*Water molecules are not included.