3ABS

Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with adeninylpentylcobalamin and ethanolamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ethanolamine ammonia-lyase heavy chain	polymer	453	49447.8	2	UniProt (P0AEJ6) Pfam (PF06751)	Escherichia coli	Ethanolamine ammonia-lyase large subunit
2	B, D (B, D)	Ethanolamine ammonia-lyase light chain	polymer	306	33197.0	2	UniProt (P19636) Pfam (PF05985)	Escherichia coli	Ethanolamine ammonia-lyase small subunit
3	E, K (A, C)	CO-(ADENIN-9-YL-PENTYL)-COBALAMIN	non-polymer		1537.6	2	Chemie (COY)
4	F, L (A, C)	ETHANOLAMINE	non-polymer		61.1	2	Chemie (ETA)
5	G, H, I, J, N... (A, C)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
6	M (C)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	P, Q, R, S (A, B, C, D)	water	water		18.0	660	Chemie (HOH)

Sequence modifications

B, D: 1 - 295 (UniProt: P19636)

PDB	External Database	Details
Met -10	-	expression tag
Asp -9	-	expression tag
Gln -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165289.7
Non-Polymers*	Number of molecules	11
	Total formula weight	3846.1
All*	Total formula weight	169135.7

*Water molecules are not included.